ICA、RIA和ELISA法测定甲胎蛋白的比较

Determination of serum α-fetoprotein by ICA, RIA and ELISA: a comparative study

目的比较测定甲胎蛋白（ＡＦＰ）的３种方法：磁粒子捕获免疫发光法（ＩＣＡ）、放射免疫法（ＲＩＡ）与酶联免疫吸附法 （ＥＬＩＳＡ）。方法 分别进行线性、精密度、对比试验比较。结果３种方法曲线相似但线性范围不同，ＩＣＡ法最宽。精密度 试验中ＩＣＡ法批内为２．５％，批间为５．８％；ＲＩＡ法批内为５．０％，批间为９．２％；ＥＬＩＳＡ法批内为１０．３％，批间为１４．６％。比 较试验显示ＲＩＡ与ＩＣＡ的结果相关性比ＲＩＡ与ＥＬＩＳＡ好。结论ＩＣＡ法测定ＡＦＰ优于ＲＩＡ和ＥＬＩＳＡ法。

Objective: To assess the efficacy of magnetic particle capture immunochemical luminescent assay (ICA) for quantative determination of α-fetoprotein (AFP) in comparison with radio immuno assay (RIA) and enzme linked immunoabsorbent assay (ELISA). Methods Thirty serum samples from randomly selected patients were collected to test AFP content with ICA, RIA and ELISA respectively. Results The linear range of AFP, was 1 to 950 ng/ml for ICA, 5 to 400 ng/ml for RIA and 5 to 300 ng/ml for ELISA. The intraassay coefficients of variation (CV) and interassay CV were 2.5% and 5.8% for ICA, 5.0% and 9.2% for RIA, and 10.3% and 14.6% for ELISA, respectively. Correlation tests revealed that the results of RIA was more closely correlated with that of ICA than with that of ELISA. Conclusion ICA is shown to be a better method for AFP quantitation than an and ELISA in terms of precision and linear range.