五味子乙素防护晶状体氧化损伤的体外实验研究

An experimental study on protection of schisandrin B against oxidative damages of lens in vitro

目的 探讨五味子乙素 (SchB)对实验性晶状体氧化损伤的防护作用。方法 采用Fenton反应复制兔晶状体氧化损伤模型 ,以吡诺克辛钠滴眼液 (PS)为对照 ,测定晶状体SP ,SOD ,GSH Px ,GSH ,VitC及MDA的含量 ,观察终浓度 0 .5mmol·L-1SchB对晶状体氧化损伤的防护作用。结果 SchB组与Fenton对照组比较 ,SchB可显著减少Fenton反应中SP的丢失 ,显著提高SOD和GSH Px的活性及GSH和VitC的水平 ,并降低MDA含量。SchB组与PS组比较 ,SchB组的SOD活性、GSH含量和VitC水平分别是PS组的 1.6 6倍 ,2 .5 8倍和 2 .36倍 ,MDA含量比PS组下降 2 4%。上述各项指标均有显著性差异 (P <0 .0 1)。结论 SchB对Fenton反应中晶状体的氧化损伤有明显的防护作用 。

OBJECTIVE: To investigate the protection role of schiandrin B against experimental oxidative damages of lens. METHODS: 20 New Zealand rabbits (40 eyes) were divided into four groups: (1)control group, (2) Fenton group (Fenton), (3) pirenoxine sodium group (PS), (4)0.5 mmol·L-1 schisandrin B group (Sch B). All fresh lenses except the control group were bathed in Fenton reaction system consisting of H2O2 and FeCl3 as a model of oxidative damages of lens. Meanwhile PS and Sch B were added into the reaction system in PS and Sch B groups respectively. Lenses were incubated in CO2 incubator for 24 hours. Then soluble protein(SP), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), Vitamin C (Vit C) and Malondiaoldehyde (MDA) in homogenized lenses were measured. RESULTS: (1) Sch B group Compared with Fenton group: SP of lens in Sch B group was significantly higher than that in Fenton reaction system (P less than or equal 0.01); The activities of SOD and GSH-Px and the levels of GSH and Vit C in the lens of Sch B group increased; MDA decreased. (2)Sch B group Compared with PS group:SOD activity in Sch B group was 1.66 times the level of PS group;GSH in Sch B group was 2.58 times the level of PS group; Vit C,2.36 times; MDA decreased 24% (P less than or equal 0.01). CONCLUSION: The findings showed that Sch B protected lens against oxidative damages in Fenton reaction system remarkably, and the anti-oxidative role of Sch B was better than PS. The results provided scientific basis for using Sch B as a potential drug to prevent and treat cataract.