高效液相色谱法测定养生宝胶囊中大黄素的含量

Determination of emodin in Yangshengbao capsule by HPLC

目的 :建立养生宝胶囊中大黄素含量测定方法。方法 :用高效液相色谱法测定 ,采用 YWG- C1 8色谱柱 (4 .6 mm× 2 5 0mm ,5︼m ) ,流动相 :甲醇 - 0 .2 5 %磷酸 (80∶ 2 0 ) ,检测波长 :λ=436 nm ,流速 :1.1m l/ min,柱温 :室温。结果 :本法简便、灵敏、准确、重现性好 ,线性范围 36～ 180︼g (r=0 .9999) ,平均回收率 99.14% ,RSD为 0 .81%。结论

AIM: To establish a method for quality control of Yangshengbao capsule. METHODS: The determination was carried out by high performance liquid chromatography with column: YWG C 18 (4 6 mm×250 mm, 5 μm), mobile phase: methanol 0 25% phosphoric acid (80∶20), detection wavelength: 436 nm, flow rate: 1 1 ml/min and column temperature: room temperature. RESULTS: This method was convenient, sensitive and accurate. The linear range was 36-180 μg. ( r = 0 999 9) and recovery rate was 99 14%, and RSD was 0 81%. CONCLUSION: The method can be used as standard quality control method.