蒺藜全草中总皂苷总含量的测定

Determination of the content of total saponins in herba tribuli

目的 :测定蒺藜全草中总皂苷的含量。方法 :将对照品蒺藜皂苷 FSA- 1和蒺藜总皂苷分别溶于硫酸 -甲醇 (7∶ 3)溶液中 ,于 6 0℃作用 15 m in后 ,用分光光度法测定蒺藜总皂苷的含量。结果 :在波长 319nm处有最大的吸收峰 ,浓度与吸收值之间有良好的线性关系。结论 :通过测定 6批蒺藜全草中总皂苷的含量 ,表明了本方法简单 ,重现性好 。

AIM: To determine the content of total saponins in Herba tribuli (Tribulus terrestris L). METHODS: UV spectral method was used. The samples or FSA 1 reacted with the solution of sulfuric acid methanol (7∶3) at 60 ℃ for 15 min. RESULTS: The detective wave length was 319 nm. The method showed good linearity. CONCLUSION: This method is used as a quantitative analytical method for six bundles of Herba Tribuli. It is simple sensitive and accurate.