凝血酶原时间测定标准化的几点探讨

Exploration of the standardization of prothrombin time

目的 探讨不同试剂、报告方式对凝血酶原时间（PT）测定结果的影响，以及标本自身因素对仪器测定PT的影响。 方法 采用两种不同国际敏感度指数（ISI）试剂，测定正常、异常两组标本，结果用秒数、凝血酶时间比值（PTR）、国际标准化比率（INR）表达，对检测结果做统计学分析。另用梯度溶血、黄疸、脂血标本观察仪器测定PT的干扰情况。 结果 两种试剂检测抗凝疗法病人血浆时，秒数和PTR差异显著（P＜0．05），而用INR表达的结果则无显著差异（P＞0．05）。中度以上的溶血、黄疸、脂血标本干扰仪器测定。 结论 监测抗凝药物治疗时，应用INR方式报告PT值，尽量采用ISI接近1．0的试剂，中度以上的溶血、黄疸、脂血标本最好用手工法测定。

 Objective To study the influence of different reagentsreport methods and the sample itself on the measurement of prothrombin time PT． Methods Two reagents with different ISI were used to test the normal and abnormal samples．The outcome inseconds was illustrated by PTR or INRand analyzed statistically． Meanwhile samples taken from patients with haemolysisjaundiceand lipemia various consistencies were applied to watch the interference measured by thrombus stanch analyzer． Resulte In measuring the plasma of the patients who have taken anticoagulant with two reagents． the difference bfetween the results reported in the form the second and PRT are significantP＜0．05 while in the case of INR not P＞0．05． And samples from haemolysisjaundice and lipemia with consistency over medium level influenced the measurement of the apparatus． Conclusion In surveying anticoagulant treatment INR should be applied to report PT and the reagent ISI chosen was approximately 1．0 Haemolysisjaundice and lipemia samples of medium consistency or above have to be measured manually． 