肝癌组织中bcl-2基因断裂点和其蛋白检测的意义

THE SIGNIFICANCE OF DETECTION OF bcl-2 GENE BREAKPOINT AND ITS PROTEIN IN HEPATOCARCINOMA

目的 探讨bcl 2基因与肝癌发生的关系。方法 采用免疫组化和核酸分子原位杂交技术原位检测 5 4例肝癌中bcl 2蛋白表达和bcl 2基因的两个断裂点。结果 40例肝细胞肝癌中 6例bcl 2蛋白阳性 ,阳性率为 15 % ,发生bcl 2基因断裂有 8例 ,阳性率为 2 0 % ,mbr和mcr阳性各 4例 ;14例肝胆管癌中bcl 2蛋白阳性 10例 ,阳性率为 71 4% ,未发现bcl 2基因断裂 ,mbr和mcr均阴性。结论 提示bcl 2蛋白异常表达可能与肝胆管癌发生关系较密切 ,而在肝细胞癌发生过程中的作用有限。bcl 2基因断裂可能与肝细胞癌发生有一定关系 ,而与肝胆管癌无明显关系。bcl 2蛋白表达与bcl

Objective To investigate the relationship between bcl-2 ge ne and hepatocarcinogenesis.Methods Bcl-2 protein expression and bcl-2 gene breakpoint were detected in 54 cases of hepatocarcinoma by immuno h istochemistry and in situ hybridization technique. Results In 4 0 cases of hepatocellular carcinoma (HCC), 6 cases were positive for bcl-2 pro tein staining (15%). Bcl-2 gene breakpoints were found in 8 cases (20%), 4 case s in mbr site and 4 cases in mcr site. In 14 cases of cholangiocellular carcinom a , 10 cases were positive for bcl-2 protein expression (71.4%) and no bcl-2 bre akpoint was found. Conclusion The results suggest that bcl-2 p rotein overexpression may be closely realted to the genesis of cholangiocellular carcinoma and does not play an important role in the pathogenesis of HCC. Bcl- 2 breakpoint may be associated with HCC genesis and has no correlation with chol angicellular carcinoma. No correlation is found between bcl-2 protein expressio n and bcl-2 gene breakpoint.