不同频率电针对大鼠尾壳核头部一氧化氮合酶表达的影响

Influence of Electroacupuncture at Different Frequencies on Nitric Oxide Synthase Expression in the Head of Nucleus Caudatus and Putamen in Rats

低频 (2Hz)和高频 (1 2 8Hz)电针大鼠“合谷”穴 ,采用还原型尼克酰胺嘌呤二核苷酸脱氢酶 (NADPH d)法 ,显示不同频率电针对尾壳核头部各区一氧化氮合酶 (NOS)表达的影响。结果表明 ,低频和高频电针均可使尾壳核头部NOS表达增强 。

Objective: To study the effect of electroacupuncture (EA) of “Hegu” (LI 4) at different frequencies on nitric oxide synthase (NOS) expression in the nucleus caudatus and putamen in the rat. Methods: 18 SD rats were randomly divided into control group (n=6), 2 Hz EA group (n=6) and 128 Hz EA group (n=6). Unilateral “Hegu” (LI 4) was punctured and stimulated with electrical pulses (3 V, duration of 1 ms, 2 Hz and 128 Hz) for 30 min. Changes of NOS expression were displayed by using nicotinamide adenosine dinucleotide phosphate (NADPH d) method and observed under microscope. Results: In comparison with control group, the number of NOS positive neuron in the dorsal lateral, ventral lateral and dorsal medial regions of the nucleus caudatus and putamen were increased significantly in both 2 Hz EA group and 128 Hz EA group (P<0.05). The fact suggests that nitric oxide of this nucleus may participate in acupuncture analgesia. Conclusion: Both 2 Hz and 128 Hz EA of “Hegu” (LI 4) can induce up regulation of NOS expression in nucleus caudatus and putamen in the rat.