管碟法与HPLC法测定盐酸环丙沙星软膏含量的比较

The comparison of determination of ciprofioxacin hydrochloride ointment by microbiological assay and HPLC method

目的 :建立了盐酸环丙沙星软膏的微生物二剂量含量测定方法 ,并与 HPL C法进行了比较。方法 :微生物法测定条件为 :检定菌 :枯草芽孢杆菌 ,培养基 :II号培养基 (p H 7.8) ,缓冲溶液 :磷酸盐缓冲液 (p H7.8) ,剂距比为 2 :1,剂量反应直线方程的范围在 0 .5～ 5 .0μg/ ml,r=0 .9993。结果和结论 :两种含量测定方法具有可比性 。

OBJECTIVE:A microbiological cylinder plate assay for ciprofioxacin Hydrochloride ointment was successfully established,and the determination of ciprofioxacin Hydrochlofide ointment by microbiological assay and HPLC method was compared. METHOD: microbiological cylinder plate assay experiment condition :medium:medium Ⅱ(pH7.8) ,test organism:bacillus subtilis,buffer:phosphate buffer (pH7.8),ratio of high concentration and low concentration solution:2:l,the linearity ranges were 0.5～ 5.0μg/ml, r=0.9993.RESULTS and CONCLUSION:It was found that the results obtained by microbiological cylinder plate assay and by HPLC methods were nearly equivalent, both methods were suitable for the determination of ciprofioxacin Hydrocldoride ointment.