生长抑素对人球后成纤维细胞增殖的影响

The Influence of Somatostatin 14 on the Proliferation of Human Retroocular Fibroblasts

目的 探讨生长抑素 1 4 (SS1 4 )治疗 Graves眼病的机理 ,为临床应用提供实验依据。方法 培养人球后成纤维细胞 (hum an retro- ocular fibroblast,HROFb) ,用 MTT比色法测定细胞增殖状况 ,并观察 SS1 4 对HROFb的生长、增殖的影响。结果 所测光吸收值与成纤维细胞的数目呈正相关 (r=0 .996,P<0 .0 0 1)。SS1 4 在低浓度下 (<0 .2 5 nmol/ L)可促进 HROFb 增殖 ,当浓度 >2 5 nm ol/ L 时则可抑制 HROFb的增殖 ,且具剂量效应关系。HROFb的增殖率与 SS1 4 浓度呈负相关 (r=- 0 .90 5 4,P<0 .0 0 1)。结论 生长抑素对人成纤维细胞增殖有双相作用。

Objective To elucidate the possible mechanism of Somatostatin 14 in the treatment of Graves' ophthalmopathy, thus providing laboratory data with the intent to underpin therapeutic developments. Methods The human retro ocular fibroblasts (HROFb) was cultured and MTT colorimetry assay was used to determine the proliferation of HROFb. Results MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the numbers of the cells ( r=0.996,P <0.001). Somatostatin 14 could enhance the cell proliferation percentage at lower levels (<25nmol/L) and inhibit that at higher levels (>25nmol/L). The cell proliferation correlated positively to Somatostatin 14 levels significantly ( r=-0.9054, P <0.001). Conclusion Somatostatin 14 has a dual effect on HROFb.