海因酶产生菌的微孔快速筛选法

The Millipore Rapid Screening of Hydantoinase Production Strains

目的 建立微孔快速筛选法以筛选海因酶活力较高的菌株。方法 比较底物对羟基苯海因的多种有机溶剂对酶反应的影响。结果 筛选出一株酶活力较高的海因酶产生菌腐臭假单胞菌 95 0 5 (Pseudomonasputida95 0 5 )。发现吐温 - 80 (1% )能很好溶解底物 ,促进酶反应。结论 为从大批量菌株中快速筛选出较高酶活力的菌株提供了一种新的方法。

Objective To find a strain with a higher hydantoinase activity. Methods The millipore rapid screening method was established to select the strains. The effects of several solvents on the rate of biotransformation were compared.Results A hydantoinase production strain has been selected by the millipore rapid screening method. Tween 80 (1%) is found to be a suitable solvent to dissolve the substrate. Conclusion A new method is provided for rapid selection of hydantoinase production strains from large scale samples. The difficulty in dissolving the substrate has been resolved to some extent.