门静脉部分结扎后大鼠血肿瘤坏死因子和内皮素与高动力循环综合征的关系

Tumor necrosis factor and endothelin and hyperdynamic circulatory syndrome in partial portal-vein ligated rats

目的 探讨肿瘤坏死因子和内皮素在高动力循环综合征 (HCS)形成中的作用。方法 门静脉部分结扎大鼠形成稳定的HCS后 ,检测用抗肿瘤坏死因子抗体处理前后各组的门静脉血肿瘤坏死因子及内皮素含量 ,同时检测血流动力学。结果 门静脉部分结扎后 0 5h ,大鼠门静脉血肿瘤坏死因子含量无变化 ,2 4h则显著增高 ,此后保持升高状态 ,并与HCS启动的时间相一致 ;门静脉血内皮素含量在门静脉部分结扎后各时相均有轻度升高。在注射抗肿瘤坏死因子抗体后 ,门静脉血肿瘤坏死因子含量明显降低至对照水平 ,内皮素含量无变化 ,HCS有部分缓解。结论 肿瘤坏死因子可能与HCS的启动有关 ,可能是维持HCS的媒介 ;内皮素与HCS形成无直接因果关系 。

Objective To study the effect of tumor necrosis factor(TNF) and endothelin(ET) on the formation of murine hyperdynamic circulatory syndrome(HCS).Methods Circulation stable HCS model was produced in rats by partial portal vein ligation.Portal blood TNF and ET was determined pre and post injection of anti rat TNF and hemodynamics was monitored simultaneously.Results TNF level did not change 0 5 hour after partial portal vein ligation,but it increased significantly at 24 hour and maintained at this high level thereafter. ET was on a small increment at different time after partial portal vein ligation.The TNF level decreased significantly to the level in controls and ET remained unchanged after injection of anti rat TNF,while HCS was partially ameliorated. Conclusion [WT5”BZ] TNF may be related with the start and maintenance of HCS,and there is no causality between ET and the development of HCS.