黏附分子在Graves眼病眼外肌原位表达的研究

A study of adhesion molecule expression in extraocular muscles of Graves′ ophthalmopathy

目的 研究Graves眼病患者眼外肌中胞间黏附分子 1(intercellularadhesionmolecule 1,ICAM 1)和血管细胞黏附分子 1(vascularcelladhesionmolecule 1,VCAM 1)的表达。方法 采用免疫组织化学双重染色方法对 16例严重Graves眼病患者的眼外肌冰冻组织切片进行光学显微镜观察。结果 ICAM 1在眼外肌束膜结缔组织、单核细胞、成纤维细胞和血管内皮细胞中表达 ;VCAM 1在血管内皮细胞中表达 ;眼外肌细胞无该两种黏附分子的表达。结论 ICAM 1和VCAM 1参与Graves眼病的病理发生过程 ;成纤维细胞、血管内皮细胞和单核细胞在自身免疫反应中起着重要作用。

Objective To investigate the expression of intercellular adhesion molecule 1 (ICAM 1) and vascular adhesion molecule 1 (VCAM 1) in extraocular muscles (EOM) of the eyes with Graves′ opthalmopathy (GO). Methods Seventeen EOM specimens obtained from 16 severe patients with GO and their cryostat sections were observed by double labeling immunohistochemical methods. Results The expression of ICAM 1 was observed in interstitial and perimysial connective tissue surrounding EOM fibers, mononuclear cells, fibroblasts and vascular endothelial cells. Only were vascular endothelial cells stained for VCAM 1, and ICAM 1 and VCAM 1 immunoreactivities were not detected in EOM cells. Conclusions The findings indicate that ICAM 1 and VCAM 1 might be involved in the development of GO, and fibroblasts, vascular endothelial cells and mononuclear cells might play important roles in the autoimmune reaction.