转化生长因子β1对体外培养口腔鳞癌细胞系尿激酶u-PA系统活性的影响

Transforming growth factor beta 1 differently modulate activity of urokinase plasminogen activator and plasminogen activator inhibitor-1 in oral squamous cell carcinoma and metastasis carcinoma cell lines.

目的 :探讨TGF - β1和口腔鳞癌侵袭转移的关系。方法 :采用发色底物反应法测量不同浓度TGF - β1作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后u -PA和PAI- 1的活性。结果 :不同浓度的TGF -β1作用于TSCCa 12h后 ,u -PA和PAI- 1活性与对照组相比 ,无明显差异 (P >0 .0 5 ) ;而不同浓度的TGF - β1作用于GNM细胞 12h后 ,u -PA和PAI- 1活性与对照组相比 ,有显著性差异 (0 .0 1<P <0 .0 5 ) ,u -PA和PAI - 1活性与TGF - β1有剂量依赖关系。结论 :TGF - β1可能与口腔鳞癌侵袭转移有关。

Objective:To detect the role of transforming growth factor β1(TGF-β1) on the invasiveness and metastasis of human oral squamous cell carcinoma.Methods:Using chromogenic substrate assay to determine the activity of u-PA and PAI-1 in oral squamous cell carcinoma cell lines TSCCa and metastasis carcinoma cell lines GNM treated by different levels of TGF-β1.Results:TGF-β1 had no effect on the activity of u-PA and PAI-1 in TSCCa cell lines;While TGF-β1 had increased the activity of u-PA and PAI-1 in GNM cell lines,Moreover,the activity of u-PA and PAI-1 had more dependent on TGF-β1.Conclusions:TGF-β1 may be a factor associated with invasive and metastasis potential of oral squamous cell carcinoma. [