鸡大肠杆菌多价灭活苗的研制

Development of inactivated polyvaccine of avian Escherichia coli

从福建省几个主要养鸡地区发病鸡场的病、死鸡的内脏和死胚中分离到的大肠杆菌 ,对其进行了生化试验、致病性试验和血清型鉴定。选择血清型为 O78、O2 、O1 和 O36 的 4个代表菌株 ,以油乳剂、蜂胶和铝胶为佐剂 ,按一定比例配制成每毫升含 10 0亿个大肠杆菌的多价灭活苗。经现场试验证明 ,该疫苗安全、有效 ,其中油乳剂灭活苗的保护期比蜂胶灭活苗长 ,但蜂胶灭活苗的抗体水平上升比油乳剂苗快 ;

Several strains of Escherichia coli were isolated from the dead chicken embryoes and the viscera of chicken either ill or dead from some breeding chicken farms in Fujian province.The isolated E coli were tested for their biochemical features,pathogenicities and serotypes.The serotypes O 78 ?O 2?O 1 and O 36 of E coli were determined to be the dominant,which were prepared for oil emulsion,propolis and aluminium adjuvant inactivated polyvaccine respectively,in which the count of E.coli was ten billion.The experiment showed that the polyvaccines were safe and effective.Compared with propolis\|inactivated polyvaccine,the oil emulsion\|inactivated polyvaccine had a longer retention period for absorption,but propolis\|inactivated polyvaccine was faster to reach the peak of antibody level than oil emulsion\|inactivated polyvaccine.The efficiency of aluminium adjuvant\|inactivated polyvaccine was inferior to propolis\|inactivated polyvaccine and oil emulsion\|inactivated polyvaccine.