PVYCP基因导入加工型马铃薯甘农薯1号的研究

STUDIES ON THE TRANSFORMATION METHODS OF PVY CP GENE TO A NEW PROCESSING POTATO STRAIN“GANNONG POTATO 1”

利用整合有马铃薯Y病毒外壳蛋白基 (PVYCP基因 )的改建质粒PEY3,以土壤农杆菌为载体转化加工型马铃薯新品种甘农薯 1号叶盘 ,细菌侵染 14d后用 10 0ml/LKM在愈伤组织水平上选择 ,转化率最高可达 2 1 3%。转化效率与细菌生理状态、NAA浓度、叶盘预培养天数有关。转化体分化 2株再生植株。冠瘿碱分析证明转化体整合了PVYCP基因。

In this study,we used the disarmed Ti plasmid PEY3 integrated potato virus Y coat protein(PVY CP)gene mediated by Agrobacterium tumefaciens to transfer the leaf disc of a new processing potato strain“Gannong potato 1”.The transformed calli were selected in the medium with KM=100 mg/L after they were induced 14 days.The result showed that the following factors affected the transformation efficiency:the bactetium physiology state,NAA concentration and the explant precultrue days.The highest transformation rate was 21 3%.Two plants were regenerated from the KM resistant calli.The opine assay was positive,which demonstrated that the KM resistant plant were integrated with PVY CP gene.