rhEGF滴眼液促进兔眼角膜内皮细胞损伤修复的实验研究

Effects of rhEGF eyedrops on endothelial wound healing of rabbit cornea in vivo

观察 rh EGF滴眼液对兔角膜内皮细胞损伤修复的作用。方法 :36只新西兰白兔机械性破坏双眼内皮细胞 ,每只兔的两侧眼随机分为实验组和对照组。实验组点用 10 0μg/ml的 rh EGF滴眼液 4次 /日 ,对照组点用赋型剂 4次 /日。在选定时间分别观察内皮损伤愈合率、角膜厚度、内皮细胞密度及形态 ,并作内皮细胞有丝分裂的测量。结果 :1术后第 2、 4天 ,实验组的内皮损伤愈合率明显快于对照组 ;第 7天两组的内皮损伤区域几乎完全愈合。 2术后第 7天实验组的角膜厚度明显小于对照组 ,第 10、 14天两组无明显差异。 3术后第 14天和第 2 1天实验组的角膜内皮细胞密度明显高于对照组。 4实验组3H- HDR标记的细胞核数明显多于对照组。结论 :兔角膜内皮细胞损伤后局部点用 rh

Objective: To investigate the influence of rhEGF eyedrops on rabbit corneal endothelial wound healing in vivo. Methods: The mechanic endothelial wounds were made in 35 rabbits. For each rabbit, one cornea was treated with 100 μg /ml rhEGF eyedrops qid, while the mate served as control treated with vehicle qid. At selected times after injury, the corneas were evaluated for corneal thickness, the rate of endothelial wound closure, the endothelial cell density and endothelial cell mitosis. Results: 1. Two days, four days after injury, endothelial wounds of eyes treated with rhEGF eyedrops had healed more rapidly than those treated with vehicle. At days 7 after injury, the sounds were almost completely resurfaced in both groups. 2. At days 7 the corneas of rhEGF-treated group were significantly thinner than those of control group. While days 14 and 21 the corneal thickness of both groups had no significant difference. 3. At days 14 and 21 after injury, the central endothelial cell ,density of rhEGF-treated corneas was significantly higher than that of the control eyes. 4. The corneas treated with rhEGF had more endothelial cell nuclei labeled with 3H-TDR compared with the paired control eyes, with significant difference. Conclusions: The local applying of rhEGF eyedrops after an endothelial cell injury significantly enhanced the healing of rabbit corneal endothelial wounds.