等位基因特异性扩增酶联免疫法检测CYP1A1基因多态性

Detection polymorphism of CYP1A1 gene by allele specific amplification ELISA

目的 建立一种高效的基因多态性检测方法。方法 用等位基因特异性扩增 (ASA)和等位基因特异性扩增酶联免疫法(ASA ELISA)对CYP1A1基因m2多态性进行检测。结果 两种方法的检测结果基本一致 ,仅有 1例不相同。ASA ELISA将地高辛标记在扩增产物上 ,然后通过酶联免疫法检测扩增产物 ,检测灵敏度与等位基因特异性扩增 (ASA)后琼脂糖凝胶电泳相比高 10 0倍。结论 该方法具有操作简单、快速、灵敏度高等特点 ,可用于大标本量的基因多态性检测。

Objective\ For establishing a effective method of gene polymorphism. Methods \ Polymorphism of CYP1A1 were detected by allele specific amplification and allele specific amplification ELISA in this study.Results\ The results of two testing methods were same except one sample.PCR products were labeled with Dig in ASA ELISA,then the PCR products were detected by specific and sensitive ELISA.The assay detection was 100 fold more sensitive than gel detection of ASA PCR products.Conclusion\ It was simple and short time,but it was more expensive.