摘要
GeneticEngineeringofTobaccowithDoubleResistancetoBothVirusandInsectLIANGXiao-you;(梁晓友)MIJing-jiu;(米景九),PanNai-sui(潘乃隧),CHENzh...
Expression vector pE14 with double resistance to virus and insect was constructed by inserting CW-cp gene and Bt-toxin gene one by into T-DNA of the same binary vector pE3. Tobacco was then transformed with Agrbacterium tumefaciens (At)GV311-SE carrying PE14. Nopaline assay and PCR amplification confirmed that both CW-cp gene and Bt-toxin gene had been introduced into tobacco genome by T-DNA of PE3. Test attack with virus and demonstrated, in some of the transgenic plants, double resistance to both infection by CMV and damage by Manduca sexta.
关键词
烟草
农杆菌
转基因
载体构建
病毒
虫害
双抗性
Coiistruction of vector
Transformation of tobacco
Double resistance to virus and insect