摘要
The presont study was carried out to investigate the effect of different amounts of gossypol on bovineblastocyst attachment and trophoblastic outgrowth in vitro. Bovine oocyte were collected from theovaries of slaughtered cows and were matured and fertilized in vitro. Cleaved oocyte were culturedin CRlaa + BOEC and TC-199 + 10% FCS combined in an 1:1 ratio. After 8 days of co-culture,the hatched blastocysts were randomly allotted to different treatment groups. All were cultured ona fetal fibroblast monolaycr (prepared from bovine fetuses) in TC-199 culture medium supplementedwith 10% fetal calf serum (TCFCS). But the groups differed from one another in the dose ofgossypol given: 0.01 μg, 0.1 μg, 1 μg, 10 μs/ml, and no gossypol as control. All cultures wereperformed in 24-well culture plates at 39℃ with 5% CO_2 in air. The results indicate that the ratesof attached and outgrowing blastocysts in the medium containing 1 μg/ml gossypol were significantlylowcr than the control group (p<0.01) and outgrowth were inhibited by gossypol in a dose-dependent manner.
The presont study was carried out to investigate the effect of different amounts of gossypol on bovine blastocyst attachment and trophoblastic outgrowth in vitro. Bovine oocyte were collected from the ovaries of slaughtered cows and were matured and fertilized in vitro. Cleaved oocyte were cultured in CRlaa + BOEC and TC-199 + 10% FCS combined in an 1:1 ratio. After 8 days of co-culture, the hatched blastocysts were randomly allotted to different treatment groups. All were cultured on a fetal fibroblast monolaycr (prepared from bovine fetuses) in TC-199 culture medium supplemented with 10% fetal calf serum (TCFCS). But the groups differed from one another in the dose of gossypol given: 0.01 μg, 0.1 μg, 1 μg, 10 μs/ml, and no gossypol as control. All cultures were performed in 24-well culture plates at 39℃ with 5% CO_2 in air. The results indicate that the rates of attached and outgrowing blastocysts in the medium containing 1 μg/ml gossypol were significantly lowcr than the control group (p<0.01) and outgrowth were inhibited by gossypol in a dose- dependent manner.
基金
Research was supported by the Rockefeller Foundation
College of Agricultural and Life Sciences,University of Wisconsin-Madion.
