摘要
Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of theinjected gene was studied using PCR technique and nonradioactive labelling methed. In a mediumof TC199 + 10% FCS, three quarters of the fertilized eggs developed to the blastocyst stage. Noapparent change of the injected gene was found before the 8-cell stage, after which it was eitherintegrated into the chromosome of the host or lost gradually. But finally, the retention rate of theinjected gene should be equal to its integration rate.
Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of the injected gene was studied using PCR technique and nonradioactive labelling methed. In a medium of TC199 + 10% FCS, three quarters of the fertilized eggs developed to the blastocyst stage. No apparent change of the injected gene was found before the 8-cell stage, after which it was either integrated into the chromosome of the host or lost gradually. But finally, the retention rate of the injected gene should be equal to its integration rate.
基金
This work was supported by National High Technoloqy Grants 86310105.
