棕色田鼠小肠肌间神经丛NOS的组织化学观察

HISTOCHEMICAL STUDY OF NITRIC OXIDE SYNTHASE IN THE MYENTERIC PLEXUS OF MICROTUS MANDARINUS SMALL INTESTINE

用NDP -黄递酶组织化学法研究了NOS在棕色田鼠 (Microtusmandarinus)小肠肌间神经丛的分布 ,同样的方法对大白鼠进行了实验比较。结果发现 ,棕色田鼠小肠肌间神经丛NOS阳性神经元形态各异 ,大小悬殊数倍 ;在神经节和阳性神经纤维中可见阳性神经元呈“串珠”状和“U”型排列。 1 0倍镜下记数 1 0 0个视野 ,观察到阳性神经元平均密度为42 8个 mm2 ,每个视野内平均有神经节 1 2 4个 ,每个神经节内平均有NOS阳性神经元 8 5个 ,占其神经节内神经元总数的 2 7 2 %。棕色田鼠为野生草食性动物 ,其NOS在小肠肌间神经丛的分布与其他杂食性啮齿类有相同之处 。

The intramural neurons containing NOS in the small intestine of the Microtus mandarinus were studied by using NADPH diaphorase histochemical method The results showed:(1)The shapes and sizes of the positive neurons were different In the ganglion and nerve strand, there existed a bread like and “U” shaped structure (2) 12 4 ganglions are in every field and 8 5 neurons in every gang lion. (3)The proportion of NOS neurons is 27 2%.(4) The density of positive neurons are about 42 8 per square millimetre.Comparing with the experimental animals such as the rats the similarity and the difference were both existed