滨麦抗条锈病基因的染色体定位和分子标记

Chromosomal Location and Molecular Marker of Resistance Gene to Puccinia striiformis West. in Leymus mollis Trin. Hara

从滨麦与普通小麦杂交后代中筛选到一条抗条锈病的小滨麦品系９３７８４。以滨麦基因 组ＤＮＡ为探针的荧光原位杂交结果表明，９３７８４是小麦与滨麦的小片段易位系，易位的滨麦染色体片段位于一对小麦染色体的短臂端部。利用该易位系构建了Ｆ２分离群体，进行Ｆ２单株 成株期抗条锈鉴定，抗性分析证明，小滨麦９３７８４中的抗条锈病基因是单基因控制的，位于滨麦染色体的易位片段上，命名为ＹｒＬｍ。进一步采用２４对Ｔａｑ Ｉ（Ｔ１～Ｔ４）／Ｐｓｔ（Ｐ１－ Ｐ６）引物组合对抗感亲本及Ｆ２分离群体进行ＡＦＬＰ分析，筛选出一个与抗条锈病基因ＹｒＬｍ连锁的ＡＦＬＰ分子标记。经克隆和测序，该标记片段长度为２０５ｂｐ，定名为 Ｐ１Ｔ３ ２０５。

The hybrid wheat line, 93784 is a stable line selected from progenies of Leymus molli Trin. Hara×common wheat, which shows a very high level of resistance to yellow rust. The results of GISH using genomic DNA of Leymus mollis as probe indicated that the 93784 was a wheat-Leymus mollis small segment translocation line, and the translocated alien chromosome fragment were located on a pair of short arm terminals of what chromosomes. Using this translocation line, we established F2 segregation population and identified its yellow rust resistance in adult stage. Genetic analysis suggested that the yellow rust resistance of 93784 was controlled by a single gene. The gene was located on the translocated chromosome segments of Leymus mollis. We called it YrLm. By AFLP analysis with TaqⅠ(T1 - T4) / PstⅠ (P1-P6) primes in individuals of F2 segregation population and their susceptive and resistant parents, we screened a molecular marker that linked to yellow rust resistance gene YrLm. We also cloned and sequenced the molecular marker. The length of the marker is 205bp, named P1T3205.