乌龟Sox基因的克隆及测序

Cloning and Sequencing of the Sox Genes in Chinemys reevesii

参照人SRY基因HMG box保守区的序列 ,设计 1对兼并引物 ,扩增了乌龟 (Chinemysreevesii)的Sox基因 ,并对扩增产物进行了克隆和测序。结果在雌雄个体中均筛选出 4个不同的Sox基因 ,无性别差异性 ;其DNA序列和编码的氨基酸序列与人相应的SOX基因的相似性分别为 92 %、 91%、 84 %、 92 %和 10 0 %、93 %、 98%、 98% ,显示出高度的保守性。

Using a pair of degenerate primers based on the conservative region,HMG-box,of human SRY gene,four different fragments were amplified from both female and male Chinemys reevesii,then cloned by using pGEM-T vector and sequenced.The sequence result indicated their high homology to human Sox genes.The identities to human Sox genes in the DNA sequence and the amino acid sequence are 92%,91%,84%,92% and 100%,93%,98%,98%,respectively.It might be concluded that Sox gene was highly conservative in phylogenesis.Our work provides molecular data for the study of sex-determining mechanism of C.reevesii,which might be temperature-dependent sex determination.