摘要
目的 :揭示脐血清在骨髓长期培养中的效应 ,为脐血清的应用提供基础。方法 :以Dexter培养法 ,观察混合脐血清 (MCBS)、组合细胞因子 (CK)在长期骨髓培养中对骨髓单个核细胞 (BMMNC)形成鹅卵石造血区 (CAFC)、长期培养起始细胞 (LTC IC)、有核细胞 (NCC)的生长。结果 :10例人骨髓 ,10 6 BMMNC培养 5周后 ,CAFC、LTC IC分别为37.1± 12 .4/ (ml.well) ,40 .9± 10 .6 (ml.well) ,NCC由接种时的 10 6 (ml.well)增至 (1.63± 0 .17) 10 6 (ml.well) ,加入10 %MCBS则可使三者得到明显扩增 ,但不及组合CK ;10 %MCBS还能明显提高组合CK对三者的扩增 ;2 0 %MCBS不能取代骨髓长期培养中的血清和组合CK对三者的扩增。结论 :MCBS中含有类似GM CSF、SCF、IL 3、IL 6、EPO等一类能使CAFC、LTC IC、NCC得到明显扩增的“活性物质”
Aim: To demonstrate the role of cord blood serum on dte long term culture of bone marrow and provide the experimental foundation for its clinical use, the response of the bone marrow mononuclear cells to the mixed cord blood serum was investigated on the basis of Dexter culture system. Methods: In ten samples of human bone mamow, 1×10 6 bone marrow mononuclear cells were planted onto the stromal cells layer and cultured for five weeks, Cobblestone area forming cells (CAFC), long term colony initiation cells (LTC IC), and the nuclear cells count in the supernatant was observed. Results: CAFC, LTC IC were 37.1±l2.4 /(ml. well),40.9±10.6/(ml.well) respectively in control group. The accounts were increased in the group of 10% MCBS, although it was less than that in mixed cytokines group. When combined with cytokines, 10% MCBS enhanced the growth of CAFC and LTC IC obviously compared with that when cytokines were used alone. 20% MCBS failed to substitute the role of mixed cytokines plus fatal calf serum on the expansion of CAFC, LTC IC and NCC. Conclusion: MCBS have the activity of stimulation for hematopoietic stem cells, which is analogous to SCF, IL 3, etc. Its nature and concentration remain to be investigated extensively.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第3期299-301,共3页
Chinese Journal of Applied Physiology
关键词
骨髓单个核细胞
混合脐血清
细胞因子
鹅卵石造血区
长期培养起始细胞
bone marrow mononuclear cells
mixed cord blood serum
cytokines
cobblestone area forming cells
long term colony initiation cells