筋膜间室综合征早期诊断的实验研究

Experimental study on early diagnosis of compartment syndrome

目的 建立筋膜间室综合征动物模型 ,以便寻找早期诊断筋膜间室综合征的方法。方法 用血压计袖带包绕犬小腿 ,充气至 80kPa压迫 12h建立实验模型。 12只犬做自身前后对照 ,动态检测血浆肌磷酸激酶 (CPK)、谷草转氨酶 (AST)、乳酶脱氢酶 (LDH)以及组织压、经皮氧分压和神经传导速度 ,松开袖带后 96h取材行病理检查。 结果 松开袖带后 12h组织压达到 7.7kPa ,此时血CPK、AST和LDH分别比正常值高 37,2 1和 12倍 ;经皮氧分压降低为正常值的 1 2 (4 .0kPa) ;神经传导速度为零 ;病理显示为不可逆变化。 结论 本研究动物模型成功、可靠 ;CPK、AST、LDH、组织压。

Objective To make a new compartment syndrome animal model to find applicable methods for early diagnosis of compartment syndrome. Methods Twelve dogs' hind limbs were pressed by an pneumatic tourniquet to make an animal model. Creatine phosphokinase,glutamic oxalacetic transaminase enzyme, lactate dehydrogenase, tissue pressure, nerve conduction velocity and PtcO 2 were monitored dynamically and the biopsy was taken 96 hours after loosening the pneumatic tourniquet. Results Tissue pressure increased to 7.7kPa 12 hours after loosening the pneumatic tourniquet, and at the same time, creatine phosphokinase, glutamic oxalacetic transaminase enzyme and lactate dehydrogenase reached their peaks, PtcO 2 declined to one half of its normal value, pathological changes of muscle and nerve were irreversible. Conclusions The new animal model of compartment syndrome is successful;creatine phosphokinase, glutamic oxalacetic transaminase enzyme, lactate dehydrogenase, tissue pressure, NCV and PtcO 2 can be used as the early diagnostic parameters for compartment syndrome.