巯基供体化合物NAC和S-8744对局灶性脑缺血/再灌损伤保护作用的比较

Protective effect of thiol donor NAC and S-8744 on focal cerebral ischemia/reperfusion damage

目的与方法 本文用插线法制作局灶性脑缺血 /再灌损伤模型 ,利用激光共聚焦扫描显微镜和fluo 3或DCF DA与PI荧光探剂双标技术检测了活体脑片细胞内脂质过氧化物、Ca2 + 含量及细胞的坏死程度。同时观察巯基供体NAC、S 8744对脑缺血 /再灌损伤的保护作用。结果 ①缺血 /再灌时脑片细胞内脂质过氧化物、Ca2 + 含量增加 ,同时脑片细胞发生坏死 ;②巯基供体NAC、S 8744均可降低缺血 /再灌损伤引起的脑片细胞内脂质过氧化物、Ca2 + 的含量 ,减轻细胞坏死程度。结论 巯基供体NAC、S 8744可拮抗局灶性脑缺血

AIM observing protective effect of thiol donor NAC and S 8744 on focal cerebral I/R damage. MOTHEDS The model of focal cerebral ischemia/reperfusion(I/R) was established in rats by reversible inserting a nylon thread, observing level of lipid peroxide,Ca 2+ and cellular necrosis in living brain slices using laser confocal scanning microscope(LCSM) with double labeling of fluorescent probes fluo 3 or DCF DA(2,7 dichlorofluorescin diacetate) and propidium iodide (PI); at the same time. RESULTS ①Increasing of lipid peroxide and Ca 2+ during I/R, meanwhile, there was necrosis in brain slice.②Thiol donor of NAC?S 8744 significantly reduces lipid peroxide and calcium content, reduced necrosis induced by I/R. CONCLUSION Thiol donor of NAC?S 8744 can effectively resist focal cerebral I/R damage.