谷氨酸对牛大脑中动脉平滑肌细胞胞浆静息Ca^(2+)和ATP触发的Ca^(2+)内流的影响

The effect of glutamate on cytosolic Ca^(2+) concentration and Ca^(2+)influx induced by ATP in cultured calf middle cerebral artery smooth muscle cells

目的 观察谷氨酸对牛大脑中动脉平滑肌细胞有无直接的激动作用以及对ATP触发的Ca2 + 内流有无影响 ?方法 采用Fura 2荧光法测定胞浆内Ca2 + 变化技术 ,在培养的乳牛大脑中动脉平滑肌细胞上观察药物的作用。结果 谷氨酸 (10～ 2 0 0 μmol·L-1)不能增加或减少细胞胞浆静息游离Ca2 + 浓度 ([Ca2 + ]i)和ATP触发的Ca2 + 内流 (谷氨酸 :10～ 40 0 μmol·L-1)。结论 谷氨酸对大脑中动脉平滑肌细胞信息Ca2 + 水平和Ca2 + 内流均无直接的影响。

AIM To investigate if glutamate has direct effects on both resting [Ca 2+ ] i and Ca 2+ influx induced by ATP in cultured calf middle artery cerebral smooth muscle cells. METHODS The effect of glutamate on [Ca 2+ ] i was determined with Fura2 fluorescence technique. RESULTS Glutamate(10～200 μmol·L -1 )didnot elevate the resting [Ca 2+ ] i level and in the concentrations of 10～400 μmol·L -1 failed to affect increase and decrese the Ca 2+ influx induced by ATP. CONCLUSION Glutamate hasn't directly effects on both resting [Ca 2+ ] i; and Ca 2+ influx in middle cerebral artery smooth muscle cells. Glutamate is unlikely to participate in regulation of cerebrovascular tention as a direct effector.