眼镜蛇毒因子引起的大白鼠胸膜渗出性炎症

Exudative pleurisy induced by cobra venom factor in rats

目的 建立旁路激活补体引起渗出性胸膜炎的模型。方法 纯化的眼镜蛇毒因子 (CVF)胸膜腔内注射 ,一定时间后检测渗出液容积、总白细胞数、蛋白浓度和组胺浓度。预先给予各种抗炎药或同时给予眼镜蛇毒另一种毒性成分直接溶血因子 (DLF) ,观察对上述指标的影响。结果 CVF0 0 8～ 2mg·kg-1注入大白鼠胸膜腔能引起有明显量效关系和时效关系的渗出性炎症 ,表现和角叉菜胶 1mg·kg-1作用类似。苯海拉明和异丙肾上腺素都能显著减少上述模型的渗出液容积、总白细胞数、蛋白含量和组胺浓度 ;吲哚美辛、地塞米松和环磷酰胺虽不影响组胺释放 ,但也可减少渗出液容积、总白细胞数和蛋白含量 ;但细胞膜补体调节蛋白CD5 9对上述的渗出性炎症无明显影响 ;而DLF能增强CVF引起的渗出性炎症。结论 CVF胸膜腔内注射能引起对常用的抗炎药敏感的渗出性胸膜炎。

AIM To establish the model of exudative pleurisy induced by alternatively activating complement and to study protection of anti inflammatory drugs on it. METHODS Purified cobra venom factor (CVF) was injected into the pleura, and then exudate volumn, total leukocyte count, protein content and histamine concentration were determined. RESULTS CVF, injected into rat pleura at dose of 0 08～2 mg·kg -1 , induced exudative inflammation in dose dependent and time dependent manner. Both diphehydramine and isoprenaline reduced significantly exudate volume, total leukocyte count, protein content and histamine concentration, while indomethacin, dexamethasone and cyclophosphamide also reduced exudate volume, total leukocyte count and protein content, although they did change the release of histamine. DLF, one of the toxic components in cobra venom, potentiated CVF induced inflammation. CONCLUSION CVF can induce significantly exudative pleurisy sensitive to anti inflammatory agents. DLF has augmentation on CVF induced inflammation.