川芎嗪和参麦注射液对脑缺血再灌注肾脏损伤老龄大鼠自由基代谢的影响

Effect of ligustrazine and shenmai injection on free radical metabolism in kidney in the aged rats after brain ischemia reperfusion

目的 从自由基损伤方面探讨川芎嗪和参麦注射液及其配伍而成的川芎参麦注射液对老龄大鼠脑缺血再灌注肾脏损伤防护作用的机制。方法 老龄 (2 0mon龄以上 )大鼠分为老龄对照组、老龄模型组、尼莫通组、川芎嗪组、参麦组、川芎参麦组 ,观察大鼠全脑缺血 30min再灌注 6 0min后肾脏组织形态和和肌酐、尿素氮、丙二醛 (MDA)含量及超氧化物岐化酶活性的变化。结果 老龄模型组肾脏出现明显的病理和功能损伤 ,用药各组肾脏组织和功能损害具有不同程度的减轻。老龄模型组肾脏组织MDA水平的增高较老龄对照组显著。川芎嗪组和参麦组MDA含量降低。结论 老龄大鼠脑缺血再灌注肾脏损伤与自由基损伤有关。各组药物对肾脏损伤均有一定的保护作用。

AIM\ To study the protective mechanism of ligustrazine(LT), shenmai injection(SI), combination of ligustrazine and shenmai injection (LSI) to injury of kidney after brain ischemia reperfusion in aged rats from the free radical change. METHODS Aged rats (more than 20 months) were divided into model group (Aged model), control group(Aged control), nimotop group(NG), ligustrazine group(LG), shenmai injection group(SIG) and LSI group(LSG). The following items was measured in aged rats with 60 min reperfusion after 30 min brain ischemia: the content of creatinine(Cr), urea nitrogen(BUN), MDA and the activitiy of superoxide dismutase(SOD). RESULTS The distinct pathological and functional injury in the kidney was found in the models, and this change was alleviated in each treatment group. The increase of MDA content in the models kidney tissue was obvious than that in the controls. The MDA level in the LG and the SIG was decreased. CONCLUSION The kidney injury after brain ischemia reperfusion in aged rats is correlated with the injury of free radical. LT, SI ,LSI and nimotop alleviate this injury. LT and SI restraine the injury of lipid peroxidation, this may be one of the mechanisms of restraining kidney injury after brain ischemia reperfusion.