摘要
目的 在体外研究新合成吡咯啉氮氧自由基及衍生物对大鼠肝细胞膜、线粒体、红细胞及卵黄磷脂脂质过氧化的保护作用 ,并探讨其作用机制。方法 采用Fenton反应法诱导大鼠肝细胞膜、肝线粒体、卵黄磷脂脂质过氧化 ,通过硫代巴比妥酸 (TBA)法测定丙二醛 (MDA)含量 ;415nm处测定H2 O2 诱导的红细胞溶血作用 ;邻苯三酚自氧化法检测其对超氧阴离子 (O·2 )清除作用。结果 带一个活性基团 (NO·)的化合物A、B可明显地抑制MDA的产生 (P <0 0 1) ,抗H2 O2 诱导的红细胞溶血作用 ,但并不影响O·2 的产生 ;具有两个活性基团 (NO·)的化合物C作用略为明显 ,IC50 <31 2 5mg·L-1;而不带活性基团 (NO·)的化合物D无抗氧化能力。结论 稳定性氮氧自由基化合物通过清除生物体系中羟基自由基 (·OH)发挥其抗脂质过氧化作用 。
AIM To investigate the anti oxidation activities of new systhesized nitroxides in liver, liver mitochondria and RBC from rats and in egg phospholipid. METHODS The homogenates of liver, liver mitochondria from rats and the suspensions of egg phospholipid were used to determine malondialdehyde (MDA) formation induced by Fe 2+ Vit C system using TBA colorimetric method. H 2O 2 caused hemolysis was measured spectrometrically. Superoxide anion was assayed spectrometrically. RESULTS Nitroxides A, B with one active group (NO·) could inhibit MDA generation caused by ·OH generation system significantly, antagonized hemolysis induced by H 2O 2, but did not affect O · 2 formation; Nitroxide C with two active group (NO·) possessed similar potent anti lipoperoxidative activities,IC 50 <31 25 mg·L -1 . But non free radical D had no effect on peroxidation of tissues and RBC. CONCLUSION Stable nitroxides exert their antilipoperoxidative effect by specifically scavenging ·OH free radicals in biological system. Trapping of ·OH free radicals by nitroxides may be accomplished by reduction of NO· group . NO·group is an essential active group.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第4期424-427,共4页
Chinese Pharmacological Bulletin
基金
甘肃省科技攻关项目资助课题NoGS982 A43 0 86
