肝硬化患者血清维生素C水平与活性氧代谢的关系

Relationship of serum vitamin C level with reactive oxygen species in patients with liver cirrhosis

目的 探讨肝硬化患者血清维生素C水平变化及其与活性氧代谢的关系。方法 对 58例肝硬化患者和 30名健康人进行血清维生素C水平与活性氧的测定和分析。测定血清维生素C水平并采用化学比色法测定血浆丙二醛 (MDA)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)含量。结果 肝硬化组血清维生素C水平显著低于对照组 (P <0 .0 1 ) ;根据肝功能Child Pugh分级 ,血清维生素C水平C级、B级 <A级。SOD/MDA低于正常 (P <0 .0 1 ) ,而MDA明显高于正常 (P <0 .0 1 )。线性相关分析显示 ,肝硬化患者血清维生素C水平与丙二醛(MDA)呈显著负相关 ( r =- 0 .57,P <0 .0 1 ) ,与SOD/MDA、GSH Px呈显著正相关 ( P <0 .0 1 ) ,r值分别是 0 .381、0 .488。结论 肝硬化患者血清维生素C水平降低 ,与肝功能损伤和活性氧代谢紊乱密切相关。

Objective To study the level of serum vitamin C (Vit.C) and to assess the relationship between Vitamin C and reactive oxyen species(ROS). Methods The plasma Vit.C levels were measured in 58 patients with liver cirrhosis and 30 normal controls by HPLC assay. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase were measured by chemocolorimetry. Results The serum Vitamin C levels in patients with liver cirrhosis were significantly lower than those in normal controls (P<0.01). So did superoxide dismutase,glutathione peroxidase and SOD/MDA ratio. On the contrary, the contents of malondialdehyde were significantly increased. According to the Child Pugh classification, the level of serum Vitamin C were significantly lower with the severity of liver cirrhosis. But the levels of serum vitamin C correlated negatively with the level of MDA. And it correlated positively with the level of SOD/MDA ratio and GSH Px. Conclusion The lowed levels of serum Vitamin C were closely associated with the severity of liver cirrhosis and metabolic disorder of the reactive oxygen species.