薄层扫描法测定大黄浸提液中游离蒽醌含量

Determination the Content of Anthraquinone Free Derivatives from Chinese Herbal Medicine by TLCS

目的 :建立大黄游离蒽醌的薄层分析方法。方法 :展开剂 :石油醚 -乙酸乙脂 -冰醋酸 (87∶ 6 .4∶ 6 ) ,展开三次 ,分别扫描。结果 :五种游离蒽醌可达到较好分离 ,芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在 0 .0 30～ 0 .15 mg;0 .0 2 6～ 0 .130 m g;0 .0 40～ 0 .2 0 0 mg;0 .0 0 2～ 0 .0 10 mg线性关系良好。结论

OBJECTIVE:To establish the TLC methods of Anthraquinone derivatives.METHODS:Using run reagent:petroleum ethylacetate acetic acid (87∶6.4∶3.6),running three times, every times scanning, respectively.RESULTS:The five components can be divided well,The linear range was Aloe emodin(0.030～0.150 mg), Rhein(0.026～0.130 mg), Emodin(0.024～0.120 mg), Chrysophanol(0.040～0.200 mg), Physcion(0.002～0.010 mg).CONCLUSION:The method is simple and accurate, and may be used as the assay method of the Anthraquainone free derivatives.