混合斑中酸性磷酸酶及精子检出时限的研究

Study on time limit of ACP and sperm positive-detected in mixed spot

目的 研究探讨性生活后活体阴道内混合斑中酸性磷酸酶（ ACP）及精子的检出时限。方法 采用取材、检验三盲法对 600例阴道拭子进行 ACP及精子的常规检验。结果 ACP最长检出时限 255h,平均检出时限 52h。精子最长检出时限 132h,精子平均检出时限 29h。 ACP检出例中阳性程度在（＋＋）以上占 41.3％，精子检出例中阳性程度为每个视野 6～ 10个占 34.3％。 结论 应用混合斑中酸性磷酸酶及精子的检出时限 ,能为侦审性犯罪案件的法医学鉴定提供客观依据。

Objective The study was carried out on time limit of ACP and sperm positive－ detected in mixed spot. Methods 600 vaginal swabs from living body after sexual intercourse were tested by routine and three－ blindness methods.Results The longest time limit of ACP which was positively detected is 255h, average time limit is 52h, 41.3％ of those samples was ACP－ detected in positive degree (＋＋ ). The longest time limit of sperm positively detected is 132h, average time limit is 29h. The number of sperms observed in 34.3％ of those samples with positive result, is from 6 to 10/HP. Conclusion This study is helpful to forensic identification of sex cases.