毒素清对大肠杆菌肺炎老龄大鼠小肠组织自由基和前列腺素代谢变化的影响

Effect of Dusuqing (毒素清) on free radical and prostaglandin metabolism of intestinal tissue in the aged rats with escherihia coli pneumonia

目的 :研究老年肺炎时小肠损伤的病理生理特点与中药毒素清的作用机制。方法 :复制大肠杆菌肺炎模型 ,分为老龄对照组、模型组、毒素清高低剂量组和氟哌酸组 5组。主要观察肺脏和小肠组织病理改变及其含水量 ,小肠组织 6酮前列腺素 F1α(6 keto PGF1α)和血栓素 B2 (TXB2 )含量 ,一氧化氮 (NO)、丙二醛 (MDA )含量及超氧化物歧化酶 (SOD)活性。结果 :肺炎时肺、小肠组织损伤明显 ,含水量增高。老龄模型组 SOD活性、6 keto PGF1α含量的降低和 NO、MDA、TXB2 含量的增高均较老龄对照组显著。与老龄模型组比较 ,毒素清用药各组 MDA和 NO含量降低 ,SOD活性和 6 keto PGF1α含量提高。结论 :前列腺素及自由基介导的损伤参与肺炎时小肠组织损伤的发生、发展。毒素清对老龄大鼠肺炎的小肠损伤改善作用明显 ,其机制与其拮抗自由基损伤和调节前列腺素代谢平衡有关。

Objective:To study the pathogenetic mechanism of intestinal injury in the aged rat with pneumonia and the effect of Dusuqing(毒素清) on it.Methods:The models of rats with Escherihia coli pneumonia were duplicated and divided into aged control group(ACG),aged model group(AMG),high doses group(HDG) and low doses group(LDG) of Dusuqing and norfloxacin group.The pathological changes in lung tissue and intestine,the contents of intestinal 6ketoprostaglandim F 1α (6ketoPGF 1α ),thromboxane B 2(TXB 2),nitride oxide(NO),malondialdehyle(MDA) and the activity of superoxide dismutase(SOD) were observed.Results:The lung and intestinal injury in AMG was more serious.The decrease of SOD activity and 6ketoPGF 1α content,the increase of content of TXB 2,NO,MDA in the AMG were more significant than those in the ACG.In comparison with those in the AMG,the level of SOD activity,6ketoPGF 1α content were increased,otherwise the contents of MDA and NO were decreased in the HDG,the LDG and the norfloxacin group.Conclusions:The injury mediated by prostaglands and free radical is related to the occurance and development of damage of intestinal tissue with pneumonia. Dusuqing is able to improve this damage obviously in aged rats with pneumonia and the mechanisms and regulation of prostaglands metabolism.