三氧化二砷对肝癌细胞增殖的影响

Study of Arsenic Trioxide on the Proliferation of Liver Cancer Cell

为探讨三氧化二砷 (As2 O3)对肝癌HepG2细胞的增殖抑制作用 ,应用台盼蓝拒染法、MTT比色法及细胞克隆形成试验 ,研究As2 O3 对肝癌细胞的增殖抑制和细胞毒作用。结果发现As2 O3 能显著抑制肝癌细胞的生长 ,5 μmol/LAs2 O3 抑制程度明显强于 1μmol/L。 5 μmol/L和 1μmol/LAs2 O3 作用后细胞克隆形成率分别为 1.5± 1.2 %和 12 3± 2 .2 % ,明显低于对照组的 30 .0± 4.2 % (P <0 .0 1)。As2 O3 对肝癌细胞有较强的细胞毒作用 ,且呈显著剂量和时间依赖性。其对于肝癌细胞的杀伤率高于 5 氟脲嘧啶 ( 5 FU )和丝裂霉素 (MMC) ,但无显著差异。As2 O3 与 5 FU及MMC存在协同效应 ,联合应用 ,对肝癌细胞的杀伤率明显升高。说明As2 O3 具有明显的抑制肝癌细胞增殖的作用 。

To explore the effect of arsenic trioxide As 2O 3 on the inhibition of proliferation of human liver cancer cell line HepG2. The morphologic changes, proliferation ability colonyforming rate of HepG2 treated by As 2O 3 were studied with light microscopy, growth curve, trypan blue and colony forming assay. The cytotoxicity of As 2O 3 on HepG2 was determined by MTT assay. The results showed that As 2O 3 could inhibit significantly the growthe of HepG2. Colony forming rate of HepG2 with 5 μmol/L As 2O 3(1.5±1.2%) was lower than with 1 μmol/L As 2O 3 (12.3±2.2%,P<0.01) and much lower than that of control group (30.0±4.2%,P<0.01). As 2O 3 had a remarkable cytotoxic effect on HepG2 and cytotoxic effect was dose and time dependent. The cytotoxic effect could be enhanced throngh the combianation of As 2O 3 with 5 FU ofr MMC. The data suggest that As 2O 3 can inhibit significantly proliferation of HepG2 and it has a potential value of the clinical therapy on liver cancer.