摘要
目的 研究亚低温对缺氧缺血脑损害 (HIBD)新生大鼠的远近期脑保护作用。方法 新生 7天的SD大鼠制作HIBD模型 ,31℃亚低温在HIBD后即刻开始干预 ,干预 6小时 ,采用原位末端标记技术检测脑组织中凋亡细胞数 ,并进行行为观察等 ,研究HIBD新生大鼠近斯脑细胞死亡和远期行为改变 ,以及亚低温对脑的远近期保护作用。结果 新生大鼠HIBD后 ,近期由坏死和凋亡导致神经元死亡 ;远期行为学无统计学意义的改变 (P >0 .1 ) ,但在饥饿诱发下 ,行为能力显著差于假手术组 (P<0 .0 5 ) ;亚低温可使近期凋亡细胞减少 ,8日龄时凋亡细胞数由皮质 47 8± 6 8,海马 37 4± 2 2 4降为皮质 3 0± 2 1 ,海马 3 3± 2 0 (P <0 .0 1 ) 。
To investinate immedicate and long term protection effect of hypothermia on brain damage in cerebral ischemia and anoxia neonatal (HIBD) rats, making 7 days neonatal rats HIBD models, hypothermia of 31° lasting 6 hours be used just after HIBD, use tunel method to detect apoptosis cells in brain tissue and behave observe method to investigate the immediate fain damage and long term abnormal behave of HIBD rats. Results shoued in the neonatal HIBD rats, apoptosis lead to neural death in recent time; their long term behavors are normal in statitcs (P>0.1) but are abnormal if starve (P<0.05); hypothermia can deduce apoptosis cells, it can deduce the number from 47.8±6.8(contex), 37.4±22.4 (hippocampus) to 3.0±2.1 (cortex), 3.3±2.0 (hippotampus) (P<0.01) when 8 days old, and be benefit to their long term behavors.
出处
《新生儿科杂志》
2001年第3期116-119,共4页
The Journal of Neonatology
