摘要
目的 :探讨胃癌组织中端粒酶活性与 DNA倍体的关系及临床意义。方法 :用端粒酶重复序列扩增法 (TRAP)检测胃癌组织中端粒酶的活性 ,用 DNA图像分析仪 (ICM)检测肿瘤细胞核 DNA倍体。结果 :胃癌组织中端粒酶的阳性表达率为 81.2 5 % ,早期胃癌病例的阳性率 5 5 .5 % ,进展期胃癌的阳性率 87.2 %。48例中异倍体肿瘤 33例 ,占 6 8.7%。 33例异倍体胃癌中 91% (30 / 33)显示端粒酶活性。结论 :胃癌组织中端粒酶活性与 DNA异倍体率有明显的相关性 ,端粒酶的表达与胃癌的病程进展有关。
Objective:To investigate the relationship between telomerase activity and DNA ploidy in gastric carcinoma.Methods:Telomerase activity was detected using telomerase repeat amplification protocol(TRAP),and image cytometry(ICM) was used to examine the DNA ploidy.Rusults:The total positive rate of telomerase in gastric carcinoma was 81.25%.The telomerase positive rate in early stage and advanced stage were 55.5% and 87.2% respectively.Aneuploid tumors tend to be telomerase positive.Conclusion:The telomerase activity is correlated with the progression and aneuploid rate in gastric carcinoma.
出处
《肿瘤研究与临床》
CAS
2001年第4期232-233,共2页
Cancer Research and Clinic
