阴阳虚证与糖皮质激素受体关系的临床与实验研究

Clinical and Experimental Studies of the Relationship Between the Weakness of Yin andYang with Glucocorticoid Receptor

目的 :探讨阴阳虚证与糖皮质激素受体 (GR)之间的关系。方法 :分别以临床典型阴虚阳虚患者、动物模型 (大鼠 )以及细胞模型 (HL- 60 )为研究对象 ,观察阴阳虚证时糖皮质激素受体 (GR)的改变 ,并探讨中药上调 GR的部分作用机制。结果 :GR水平降低是阴阳虚证发展到一定阶段的共同病理基础之一 ;阴阳虚证发展到一定阶段时 ,体内 GR下降无器官特异性 ;上调 GR是参附汤、生脉饮被用于临床急救的重要作用机理之一 ;参附汤、生脉饮上调 GR的重要作用途径之一是增加细胞内 GRm RNA的表达。结论 :GR数量和 /或活性的下降及其下降的幅度有可能作为中医虚证极其严重程度的重要微观指标之一 ;有可能从生脉饮或参附汤中筛选出一种有效上调 GR的成分 (单体或混合物 ) ,并用于临床以虚证为主要表现的一类疾病 (如肿瘤、风湿病、乃至艾滋病等 )的治疗。

Objective:To explore the relationship between the weakness of Yin and Yang with glucocorticoid receptor. Methods:Three groups were set up (patients with weakness of Yin and Yang, Rat model and HL60 cell model) to observe the changes of glucocorticoid receptor (GR) in cases with weakness of Yin and Yang and explore the possible mechanism of the raising GR levels. Results:The common pathological basis of the weakness of Yin and Yang was the lowered GR level without organ specificity. Shen-Fu decoction and Shen-Mai-Yin could raise GR level. which was widely used in emergent cases because it increased the GR mRNA expression. Conclusions:GR levels could be used as an index for the severity of weakness of Ying and Yang. Shen-Fu decoction and Shen-Mai-Yin could raise GR level and could be used to treat cancer, rheumatism and AIDS.