海门山羊超排及血浆孕酮和17β-雌二醇浓度

SUPEROVULATION AND THE PLASMA PROGESTERONE AND OESTRADIOL-17β CONCEN TRATIONS OF HAIMEN GOATS

对28只性周期正常山羊分别注射1500iu PMSG(Ⅰ组)、300iu FSH(Ⅱ组)和等量生理盐水(Ⅲ组),比较两种促性腺激素的超排效果;用放射免疫方法测定血浆孕酮(P_4)和17β-雌二醇(E_2-17β)浓度。试验发现:(1)Ⅰ、Ⅱ、Ⅲ组的排卵率依次为12.00+1.71、21.22+2.25和2.75±0.31,超排效果FSH优于PMSG。(2)PMSG和FSH处理后,P_4和E_2-17β浓度都上升,但PMSG组只有在48h后两浓度达显著水平,FSH处理只经24h就显著升高。注射PGF_(2α)后两组的P_4浓度急剧下降(ρ<0.01)。发情时P_4浓度最低,E_2-17β为最大。(3)处理当日及此后的孕酮浓度与排卵率之间无显著相关(ρ>0.05)。而发情时的E2-17β浓度与排卵率之间呈强相关(r=0.72,ρ<0.001)。

28 does, exhibiting normal estrus cycles, were divided into three groups to compare the ovarian response. Trial 1 were injected i.m. with 1500 iu PMSG. Trial 2 with 300 iu. FSH. Trial 3 with saline. The plasma progesterone (P_4) and oestradiol-17β(E_2-17β) concentrations were estimated at different time before and after superovulation to study their changing way. The results indicated that: (1) the ovulation rate (O.R.) for 3 groups was 12.00±1.71, 21.22±2.25 and 2.75±0.31 respectively. The effect of FSH treatment was better than that of PMSG in other respects also, e.g. the number of recovered embryos. (2) the P_4 and E_2-17β levels rose significantly after 48h of PMSG treatment, but only 24h after FSH injection. P_4 levels fell rapidly after PGF_(2α) injection (p<0.01) and reached the lowest at estrus. All goats had the highest levels of E_2-17β at this time. (3) there was no significant relationship between the P_4 levels and O.R. in 3 groups (p>0.05), but the top values of E_2-17β at oestrus were postively correlated with the O. R. (r=0.72, p<0.001).