CDKN2/p16基因在四种原发恶性肿瘤中存在状态的研究

Study on the existence state of CDKN2/p16 gene in human primary malignant tumors

目的 :研究CDKN2 /p16基因缺失和蛋白丢失在非小细胞肺癌、食管癌、胃癌、乳腺癌 4种原发恶性肿瘤中的作用。方法 :采用免疫组织化学法和多重PCR技术对 68例 (非小细胞肺癌 3 1例 ,食管癌 15例 ,胃癌 13例 ,乳腺癌 9例 )原发肿瘤组织标本进行CDKN2 /p16基因第一、第二外显子纯合缺失和p16蛋白表达丢失分析研究。结果 :第一或 (和 )第二外显子总缺失率为 13 .2 % ( 9/68) ,蛋白丢失率为 44% ( 3 0 /68) ,不同肿瘤组织标本的p16基因缺失率和蛋白丢失率差别很大。结论 :CDKN2 /p16基因是重要的多肿瘤抑制基因 ,在不同肿瘤组织中其失活方式不完全相同。

Objective To study CDKN2/p16 gene homozygous deletion and p16 protein loss in human primary.Methods Multiple PCR technique and immunohistochemical method were used to detect homozygous deletion of CDKN2/p16 gene exon 1,exon 2 and loss of p16 protein in 68 cases of primary cancers, including 31 non small cell lung cancers,15 esophageal carcinomas,13 gastric carcinomas and 9 breast cancers.Results Total rate of exon 1 or(and) exon 2 deletion was 13.2%(9/68).p16 protein loss rate was 44%(30/68).p16 gene deletion rates and p16 protein loss rates in various tumors were different.Conclusion CDKN2/p16 gene is an important inhibitory gene of many tumors.Mechanisms of CDKN2/p16 gene deletion are different in various tumors.