MS-PCR法评估家族性原发性高血压病人血管紧张素原基因M235T多态性

Angiotensinogen gene M235T polymorphism in familial primary hypertension using MS-PCR technique

本文采用新型高敏感、特异的MS PCR法对中国苏皖地区 94例具高血压家族史和 36例无家族史者检测血管紧张素原M2 35T基因型。具有高血压家族史的原发性高血压病人 (FH组 )、具家族史的非高血压者 (FNH组 )、无家族史的正常对照 (N组 )和无家族史的高血压病人 (NFH组 )血管紧张素原 (Angiotensinogen ,AGT)基因TT型 /T等位基因频率分别为 0 5 81/ 0 77、0 5 6 3/ 0 77、0 346 / 0 5 8和 0 4/ 0 5 5。有家族史者T等位基因频率远高于无家族史者。小样本研究认为中国苏皖地区人群原发性高血压的发病与AGTM2 35T基因多态性密切相关 ,T等位基因具更高的发病风险。各组间年龄分析结果显示AGTM2

To apply more specific MS PCR method to determine the correlation between angiotensinogen gene M235T (AGTM235T) polymorphism and the onset of familial primary hypertension in Jiangsu and Anhui Provinces The TT genotype and the frequency of T allele (TT/T) in four groups ( 62 patients with both hypertension and familial background (FH), 32 normal persons who with hypertensive familial background (FNH), 26 persons in control group (N) and 10 hypertensive patients (NFH group) who have no familial background) were 0 581/0 77?0 563/0 77?0 346/0 58 and 0 4/0 55 respectively There was a excess in the T allele ( P <0 005) in FH and FNH groups compared with the groups which have no familial background In bordland of Jiangsu and Anhui, China, the onset of hypertension has a close correlation with AGTM235T polymorphism Concerning of the age, we speculated that the AGTM235T polymorphism was only associated with genetic hypertension, not all types of hypertension