摘要
观察碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对兔晶体上皮细胞 (rabbitlensepithelialcells,RLECs)的促增殖作用 ,以及增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的表达。用第 2～ 3代培养细胞 ,用MTT法测定细胞增殖 ,用免疫组织化学法观察PCNA的表达 ,流式细胞仪观察细胞周期的变化。结果显示bFGF可促进兔晶体上皮细胞的增殖 ,尤其是浓度为 10 μg/L作用最明显 ;正常细胞组PCNA表达呈阴性 ,添加bFGF组PC NA表达呈强阳性 ;并显示进入S期的细胞明显增加。
To study the ability of basic fibroblast growth factor (bFGF) on proliferating of rabbit lens epithelial cells(RLECs), and the proliferating cell nuclear antigen(PCNA)was expressed 2～3 passage RLECs were exposed to different concentrations of bFGF, and the proliferation characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay The expression of PCNA was observed by immunocytochemical staining The changs of cells cycles were observed with flow cytometer (FCM) After the treatment of bFGF, RLECs showed the marked proliferation , especially in 10μg/L of bFGF The expression of PCNA showed positive staining ,and S phase cells obviously increased So bFGF is an important factor that can promote the mitotic activity of RLECs
出处
《基础医学与临床》
CSCD
北大核心
2001年第4期361-364,共4页
Basic and Clinical Medicine
