摘要
探讨低剂量白细胞介素 1β(IL 1β)预处理能否增强心肌在延迟相对抗缺血再灌注 (I/R)损伤。在大鼠 ,观察注入IL 1β后即刻、12和 2 4h末开始缺血 (1h)再灌注 (2h)后心肌热休克蛋白 72 (HSP72 )、IL 1β和超氧化物歧化酶(SOD)的变化 ,测定心梗面积 ,记录心功能和再灌注室性心律失常的发生率。与对照组和缺血再灌注组 (I/R)比较 ,IL 1β预处理 (ILPC)后 2 4hI/R末 ,HSP72表达增加 ,IL 1β表达减少 ,心梗面积下降 (P <0 0 5 )。与对照组比较 ,I/R和ILPC组各时相点左室舒张末压 (LVDP)均有显著升高 ,而左室内压上升和下降速率 (±dp/dt)及SOD含量显著下降 (P <0 0 5～ 0 0 1) ,但ILPC组 2 4h末的升幅和降幅均较小 (P <0 0 5 ) ,且再灌注室性心律失常的发生较I/R组明显减少 (P <0 0 5 ) ;即刻和 12h ,ILPC组与I/R组间无变化。说明低剂量IL 1β预处理可诱导心肌延迟保护作用 ,其机制与心肌氧化
To explore whether the low dose of interleukin 1β(IL 1β) pretreatment can enhance the myocardium resist ance to ischemia and reperfusion(I/R) injury during the late phase We measured the changes of heat shock protein72(HSP72), IL 1β and superoxide dismutase(SOD) of the myocardium ,determined the infarct size and recorded the change of heart function and the rate of reperfusional arrhythmia at the end of ischemia 1h and reperfuse 2h after immediately,12h and 24h after IL 1β injected rats There was a significant increase of left ventricular diastolic pressure(LVDP) and a significant decrease of ±dp/dt and SOD contents in the ILPC and I/R groups than in the control at 0～24h after IL 1β or NS pretreat,but the increased amplitude of LVDP and decreased amplitude of the ±dp/dt and SOD were significantly lower in the ILPC group than in the I/R group at the end of 24h after IL 1βpretreatment( P <0 05) At the same time,the expressions of HSP72 increased and IL 1β decreased significantly ,the infarct size reduced remarkably in the ILPC group than in the control and I/R groups ,and the rate of ventricular arrhythmia was obviously reduced in the ILPC group than in the I/R group( P <0 05) No difference was observed in the ILPC and I/R groups at immediately and 12 hours It suggested that low dose IL 1β pretreatment induced the late cardioprotective and its mechanism could be associated with increase of protective proteins and the balance of oxidants and antioxidants
出处
《基础医学与临床》
CSCD
北大核心
2001年第4期368-370,共3页
Basic and Clinical Medicine
