人参标准提取物制备工艺初探

Primary Study on Preparation Process of Standard Extracts of Panax ginseng

目的 :寻找较好的人参标准提取物制备工艺。方法 :试验利用吸附树脂分离纯化人参醇提物 ,采用D1 0 1 、AB- 8、H PD- 10 0、Dx- 5、D35 2 0 、H1 0 3、NKA - 9七种树脂 ,先用水洗脱 ,然后用 70 %乙醇洗脱 ,用分光光度法进行含量测定。结果 :总皂苷含量是 D1 0 1 >HPD- 10 0 >A B- 8>D35 2 0 >Dx- 5 >NKA- 9>H1 0 3,收率则 :D1 0 1 >AB- 8>HPD- 10 0 >D35 2 0 >Dx- 5 >NKA- 9>H1 0 3。其中较好的是 D1 0 1 、AB- 8和 HPD- 10 0树脂 ,含量可达 75 % ,收率也在 1.8%以上。结论 :D1 0 1 、AB- 8及 HPD- 10 0树脂可用于制备人参标准提取物。

OBJECTIVE:To find better preparation process of standard extracts of Panax ginseng.METHODS:Alcohol extracts of ginseng were separated and purified by seven types of macroporous resins D 101 ,AB 8,HPD 100, Dx 5, D 3520 ,H 103 and NKA 9. At first, that is eluated with water ,then eluated with water EtOH(3:7). The quantitative determination was used the spectormetry.RESULTS:The contents of total saponin were D 101 >HPD 100>AB 8>D 3520 >Dx 5> NKA 9>H 103 .The yield of total saponin were D 101 > AB 8> HPD 100>D 3520 >Dx 5>NKA 9>H 103 .The better types resins were D 101 ,AB 8 and HPD 100 macroporous resins.Their puity were over 75% and yield were 1.8% at least.CONCLUSION:D 101 ,AB 8, and HPD 100 macroporous resins can be used in preparation of standard extracts of Panax ginseng.