应用等离子体辐射技术选育柔红霉素产生菌——天兰淡红链霉菌

SELECTION OF DAUNORUBICIN-PRODUCING STRAIN S.COERULEORUBIDUS BY PLASMA RADIATION TECHNOLOGY

报道了抗肿瘤抗生素柔红霉素产生菌———天兰淡红链霉菌激光株经能量为 6 0 - 85keV ,辐射剂量为 9.6× 10 9- 1.5× 10 11/cm2 ,(辐射时间 5- 2 0s)的N+等离子体诱变处理后 ,拟合了该菌致死率 (D)与辐射剂量 (M)的关系式D =1-Ke-MP(k ,P为相关系数 ) ,该式符合辐射生物学中的单击曲线。经摇瓶筛选后 ,获得 1株高产柔红霉素突变株 137,在生产罐上应用 ,其柔红霉素效价较亲株提高了 2 5.8%

This paper reported the results of mutagenesis by nitrogen plasma radiation with energy from 65 to 80 keV and dose from 9.6×10 9 to 1.5×10 11 /cm 2 in antineoplastic antibiotics daunorubicin-producing S. Coeruleorubidus.The relationship between death rate and radiation dose was formulated by computer and the formula? was D=1-Ke-MP (K and P were correlation coefficients).It was fit to a biological single-hit curve. The obtained high-producing mutagenic strain 137 was tested for its production property.The result showed that it could increase the daunorubicin potency by 25.8% in productive tanks of fermentation.