摘要
以 6个茄子品种为试材 ,在低温胁迫条件下进行花药培养。结果表明 5~ 6℃低温胁迫处理可诱导花药形成耐冷性愈伤组织 ,在MS +NAA(0 .0 2mg·L-1) +ZT(1.0mg·L-1)培养基上耐冷性愈伤组织的分化率达到31.6 % 。
Eggplant anthers were cultured under low temperature stress for induction of chilling tolerance using 6 varieties of eggplant as experimental materials. The results showed that chilling tolerant anther callus can be induced at 5-6℃,the differentiation frequency of chilling tolerant callus was 31.6% on MS+NAA (0.02mg·L -1 )+ZT (1.0mg·L -1 ) medium,the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control varieties under low temperature stress.
出处
《中国蔬菜》
北大核心
2001年第3期7-9,共3页
China Vegetables
基金
北京市自然科学基金资助项目