M onte Carlo方法研究支气管组织自体荧光光谱

AUTOFLUORESCENCE SPECTRUM OF HUMAN BRONCHIAL TISSUE BY MONTE CARLO MODELLING

在实验获得支气管组织显微荧光特性和有关光学特性参量的基础上 ,将支气管组织简化为三层光学模型 ,用 Monte Carlo方法模拟 44 2 nm He-Cd激光在组织模型中的光分布及自体荧光的逃逸函数 ,计算出各组织层对总体自体荧光的贡献 ,并模拟出逃逸到组织体表面的自体荧光光谱 .进一步实验测量与理论计算的自体荧光光谱比较表明 ,两者在 460～ 5 2 0 nm及60 0～ 70 0

Based on tissue microscopic fluorescence properties and optical parameters,a three layer bronchial optical model was developed to calculate the excitation light (442nm) distribution in the tissue and the fluorescence escape function from the tissue by Monte Carlo simulations The contributions of each layer in the tissue to total autofluorescence signal observed at the tissue surface were also estimated Moreover,the fluorescence spectrum of the bronchial tissue was also modelled using the obtained tissue micro spectroscopic properties It is found that the theoretically modelled spectrum agrees well with the experimental in vitro tissue fluorescence in the spectral range between the 460～520nm and 600～700nm