CD28和Fas在辐射所致T细胞凋亡中作用的研究

STUDY ON EFFECT OF CD28 AND Fas IN CELL APPOPTOSIS OF INIZING RADIATION INDUCED

采用双标记直接免疫荧光—流式细胞仪分析技术 ,测定了不同剂量γ射线照射后T细胞CD2 8分子和Fas受体 (CD95 )表达的变化。用乙醇抽提法———流式细胞仪分析技术和JAM检测技术 ,研究了不同剂量γ射线照射、CD2 8单抗和IL - 18处理后T细胞凋亡的情况。结果显示 ,正常人淋巴细胞用不同剂量γ射线照射后 ,CD3+ T细胞中CD2 8的表达随着剂量的增加而下降 ,T细胞凋亡的增加并与Fas表达上升有关。照射前用CD2 8单抗和IL - 18处理细胞后 ,Fas表达下调 ,细胞DNA断裂明显减少。表明γ射线可影响T细胞CD2 8受体分子的表达及其信号转导 ,加速T细胞凋亡的进程 ,而CD2 8单抗和IL -

After irradiation with 0.5, 2, 4 and 8Gy γ rays, CD28 and Fas (CD95) molecule expression in human T lymphocytes were measured by flow cytometry(FCM) with direct immunofluorescence technique. Before and after treatment of T cells with CD28McAb and IL-18, the T cells apoptosis was determined by JAM test and FCM technique. Results showed that with the increasing radiation dosage, the CD28 molecule expression decreased and the CD95 molecule expression increased in CD3 +T cells, which could affect signal transduction and increase cell apoptosis. However, after using CD28 McAb and IL-18 to treat the cells irradiated, Fas expression and T cell apoptosis decreased.