受体细胞倍性对PVY CP基因向马铃薯遗传转化影响的研究

STUDIES ON THE EFFECT OF RECIPIENT CELL PLOIDY ON PVY CP GENE TRANSFORMATION IN POTATO

利用整合PVYCP基因的改建质粒PEY3,以农杆菌为载体向四倍体栽培“甘农薯 1号” (2n =4x =4 8)及双单倍体“84 4 7” (2n =2x =2 4 )叶盘、叶柄、茎段导入PVYCP基因并获得再生植株。愈伤组织卡那霉素 (KM )抗性筛选试验表明 ,低倍体受体细胞利于外源基因的整合与表达 ,双单倍体转化率 (2 5 4 % )高于四倍体 (15 8% )。冠瘿碱分析证明再生植株整合了PVYCP基因。染色体倍性鉴定证明转基因再生植株与亲本倍性一致。

In this study,we used the disarmed Ti plamid PEY3 integrated PVY CP gene mediated byAgrobacterium tumefaciens to transform a tertraploid Solanum tuberosome strain'Gannong potato 1'and a dihaploid Solanum tuberosome strain '84 47'.The transformed platnlet of these two strains were all regenerated. The result showed that the dihaploidy strain was easier to be transformed than te tertraploid strain.The opine assay was positive,which demonstrated that the KM resistance plantlets was integrated with PVY CP gene.The identification test showed that all the regenerated transformed plantlets were of the same ploidey as their parents.$$$$