多孔壳聚糖可降解材料的制备与生物相容性实验研究

PREPARATION OF POROUS BIODEGRADABLE CHITOSAN AND ITS BIOCOMPATIBILITY STUDY

目的: 制备多孔可降解壳聚糖材料并观察其生物相容性。方法:将壳聚糖溶于醋酸中制成1％壳聚糖醋酸溶液,加胎牛血清与0.25％戊二醛溶液搅拌,冷冻后减压干燥。取一片材料做扫描电镜观察。测量孔径大小并做图像分析,计算孔隙率。取6片埋入3只大白兔皮下,于4、6、8周后取材作常规石腊切片,HE染色,光镜观察。结果:材料表面呈多孔结构,孔径为40-130μm,孔隙率为85％。术后4周时可见材料周围有少量炎性细胞浸润,孔隙内有纤维结缔组织长人。6周和8周时包绕的纤维组织变得致密,炎性细胞减少,无材料排出现象。结论:本材料具有良好的生物相容性和多孔结构,是可供选择的组织工程材料之一。

Department of Otolaryngology, Tangdu Hospital of Fourth Military Medcal University, Xi' an, 710038; Department of Pharmacy, Tangdu Hospital of Fourth Military Medical University, Xi' an, 710038. Abstract objective To manufacture a biodegradable porous chitosan material and observe its biocompatibility. Method: Chitosan was soluted into acetic acid to get a 1% chitosan- acetic acid solution. Fetal calf serum and 0.25% glutaraldehyde were mixed with the chitosan solution. Then they were dried by a freeze dryier. The material was scaned by an electron microscope. The microscopic photos were analyzed by image pattern analysis to measure the porous size and porous rate. Six pieces of the material were implanted subcutaneously onto the backs of three rabbits. These animals were sacrificed and the , specimens were removed 4,6, 8 weeks later . The specimens were embedded in paraffin, sectioned and stained with hematoxylin and eosin. Result: Scanning electron microscopic photos showed that the surface structure of the1 specimens was porous and the diameters varied from 40 to 130 jit m. The image analysis demonstrated that,the porous rate was 85% . A few inflammatory cells were seen around the implants 4 weeks later. Fibro ?connective tissue grew into the porous. The fibrous tissue around the implant became thicker at 6 weeks and 8 weeks along with the decrease of the inflammatory cells. No infectious or rejective reaction was found. Conclusion: Chitosan scaffold had a good biocompatibility and a certain 3-D structure with porous surface, and it was an altenative material for tissue engineering.